Analysis Note

ControlRat brain tissue lysate

Application

Detect WAVE1 using this mouse monoclonal antibody, Anti-WAVE-1 Antibody, clone K91/36 validated for use in western blotting & IHC.

Research CategoryNeuroscience

Research Sub CategoryDevelopmental Signaling

Immunohistochemistry Analysis: A representative lot detected WAVE-1 in adult rat hippocampal CA1 tissue (Prof. J. Trimmer, University of California, Davis).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Protein WAVE-1 (WAVE1) is also known as Wiskott-Aldrich syndrome protein (WASP) family member 1 (WASF1), verprolin homology domain-containing protein 1, or SCAR1. WAVE1 is a downstream effector molecule involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton. WAVE1 binds actin and ARP2/3 complex. It binds the ARP2/3 complex through the C-terminal region and actin through verprolin homology (VPH) domain. WAVE1 is highly expressed in brain, with low expression in several other tissue.

Immunogen

Linear peptide corresponding to human WAVE-1.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Protein G Purified

Format: Purified

Quality

Evaluated by Western Blotting in rat brain tissue lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected WAVE-1 in 10 µg of rat brain tissue lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~ 70 kDa observed